AD - Department of Ecology and Protection of Environment, Faculty of Veterinary Medicine, Aristotle University, 541 24, Thessaloniki, Greece. kamarian@vet.auth.gr TA - Reprod Toxicol LR - 20031114 DCOM- 20031027 PST - ppublish AB - Organochlorine pesticides and polychlorinated biphenyls are widely used in agriculture and industry, respectively. The present study assessed the burden of environmental pollutants in the follicular fluid of farm animals (cattle, sheep, goats, and pigs). An analytical method combining a solid-phase extraction with (C(18)) for clean-up and GC-electron capture detection using a capillary column was implemented for isolation and determination of organochlorine pesticides and polychlorinated biphenyls (PCBs). Of the organochlorine pesticides, hexachlorobenzene (HCB), hexachlorocyclohexane (HCH, alpha-, beta-, and gamma-isomers), dieldrin, heptachlor epoxide, and the DDT-related chemicals (o,p'-DDE, p,p'-DDE, p,p'-DDD, p,p'-DDT) were detected and of the PCBs, the congeners PCB-52, -101, -138, -153, and -180 were detected. In all species of farm animals, the most frequently detected pollutant was gamma-HCH (90-100% of samples) followed by HCB (80-100%), and p,p'-DDE (75-90.91%). Species differences in the concentrations of HCB, beta-HCH, heptachlor epoxide, and DDT-related chemicals in follicular fluid were noted as well as differences in the concentrations of some pollutants within the same species. STAT- completed RN - 58-89-9 (Lindane) RN - 50-29-3 (DDT) RN - 0 (Insecticides, Organochlorine) RN - 0 (Polychlorinated Biphenyls) RN - 118-74-1 (Hexachlorobenzene) RN - 0 (Environmental Pollutants) LA - eng PG - 185-90 SB - IM DA - 20030318 IP - 2 EDAT- 2003/03/19 04:00 AU - Kamarianos A AU - Karamanlis X AU - Goulas P AU - Theodosiadou E AU - Smokovitis A JID - 8803591 OWN - NLM MHDA- 2003/10/28 05:00 PMID- 12642151 SO - Reprod Toxicol 2003 Mar-Apr;17(2):185-90. DP - 2003 Mar-Apr AID - S0890623802001181 [pii] IS - 0890-6238 TI - The presence of environmental pollutants in the follicular fluid of farm animals (cattle, sheep, goats, and pigs). FAU - Smokovitis, A FAU - Karamanlis, X FAU - Theodosiadou, E FAU - Kamarianos, A FAU - Goulas, P MH - Female MH - Sheep MH - Follicular Fluid/*chemistry MH - Swine MH - Polychlorinated Biphenyls/analysis MH - Lindane/analysis MH - Hexachlorobenzene/analysis MH - Goats MH - Insecticides, Organochlorine/analysis MH - Animals MH - Animals, Domestic/*metabolism MH - Cattle MH - DDT/analogs & derivatives/analysis MH - Environmental Pollutants/*analysis PL - United States VI - 17 PT - Journal Article